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Erhållande av primära osteocyter genom murin kalvvarial fraktionering av GFP-uttryckande osteocyter
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Erhållande av primära osteocyter genom murin kalvvarial fraktionering av GFP-uttryckande osteocyter

Article DOI: 10.3791/61513-v • 07:22 min • June 2nd, 2020
June 2nd, 2020 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Orthodontics and Dentofacial Orthopedics, Department of Translational Medicine, Tohoku University Graduate School of Dentistry

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Detta protokoll beskriver dissektion av neonatal dmp1-topaz muscalvaria och isolering av osteocyter som uttrycker det gröna fluorescerande proteinet genom cellsmältning och fraktionering, förutom osteocytberedning för fluorescensaktiverad cellsortering (FACS).

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Retraktion utgåva 160 primär osteocyt Dmp1 SOST GFP fraktionering FACS
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