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Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
JoVE Journal
Biology
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JoVE Journal Biology
Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring

Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring

DOI:
10.3791/61525-v

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09:34 min

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August 06, 2020

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, , , , , , , , , ,
1Genome Biology of Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 2Applied Genomics for Neurodegenerative Diseases,German Center for Neurodegenerative Diseases (DZNE), 3Department for Neurodegenerative Diseases, Hertie Institute for Clinical Brain Research,University of Tübingen

Chapters

  • 00:05Introduction
  • 00:43New Culture Plate and Tip Loading and Decontamination
  • 01:35Culture Medium and/or Dissociation Reagent Filling
  • 02:09Automated Method Execution
  • 02:51Human Induced Pluripotent Stem Cell (hiPSC) Seeding
  • 04:09Automated Confluence Assessment
  • 04:47Medium Change
  • 05:37Subcultivation
  • 06:42Automated High Content, High Throughput Imaging
  • 07:14Results: Representative Automated System-Produced hiPSC-Derived Cortical and Dopaminergic Neuron Characterization
  • 08:58Conclusion

Summary

Automatic Translation

Automatic Translation

We show the automation of human induced pluripotent stem cell (hiPSC) cultures and neuronal differentiations compatible with automated imaging and analysis.

Tags

Automated ProductionHuman Induced Pluripotent Stem CellsCortical NeuronsDopaminergic NeuronsLive-cell MonitoringStandardized ProceduresLow Experimental VariationHigh Phenotypic ReproducibilityGraphic User InterfaceResource Instrument ProcessHEPA HoodDecontaminationUV RadiationLiquid Handling StationCulture MediumDissociation ReagentCell LineProjectBatchSeedingBrightfield Imaging CytometerCell Counting

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