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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats
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Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

DOI:
10.3791/61532-v

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08:58 min

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August 25, 2020

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, , , , , , ,
1Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology, 2The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology

Chapters

  • 00:05Introduction
  • 01:10Harvesting Rat Mandibles
  • 02:21Isolation and Cultivation of the mBMSCs
  • 03:39Flow Cell Sorting
  • 06:24Results: Osteogenic, Adipogenic, and Chondrogenic Differentiation of mBMSCs
  • 08:10Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a method that combines whole bone marrow adherence and flow cytometry sorting for isolating, cultivating, sorting, and identifying bone marrow mesenchymal stem cells from rat mandibles.

Tags

Keywords: MandibularBone MarrowMesenchymal Stem CellsIsolationCultivationRatsCranial Facial BoneBMSCsBone Marrow AdherenceFluorescent Cell SortingMandibular BodyMandibular RamusCoracoidCondyleMarrow CavityAlpha-MEMFBSCentrifuge

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