Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Yueyang Hong; Hongyuan Xu; Yiling Yang; Siru Zhou; Anting Jin; Xiangru Huang; Qinggang Dai; Lingyong Jiang

doi:10.3791/61532

Journal

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Medicine

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Isolamento e coltivazione di cellule staminali mesenchimali del midollo osseo mandibolare nei ratti

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

JoVE Journal
Medicine

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JoVE Journal Medicine

Isolation and Cultivation of Mandibular Bone Marrow Mesenchymal Stem Cells in Rats

Isolamento e coltivazione di cellule staminali mesenchimali del midollo osseo mandibolare nei ratti

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5,126 Views

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08:58 min

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August 25, 2020

DOI:

10.3791/61532-v

DOI:

10.3791/61532-v

•

08:58 min

August 25, 2020

•

5 Views

Yueyang Hong*¹, Hongyuan Xu*¹, Yiling Yang¹, Siru Zhou¹, Anting Jin¹, Xiangru Huang¹, Qinggang Dai², Lingyong Jiang¹

¹Center of Craniofacial Orthodontics, Department of Oral and Cranio-maxillofacial Science, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology, ²The 2nd Dental Center, Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology,National Clinical Research center of Stomatology

Transcript

Summary

Questo articolo presenta un metodo che combina l'aderenza al midollo osseo intero e lo smistamento della citometria del flusso per isolare, coltivare, smistare e identificare le cellule staminali mesenchimali del midollo osseo dalle mattidi di ratto.