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Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq
DOI:

06:22 min

August 25, 2020

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Chapters

  • 00:04Introduction
  • 01:01Tissue Dissection and Dissociation
  • 03:20Gradient Centrifugation and Isolation of Nuclei
  • 04:56Results: Comparison of UMIs and Genes Obtained from Different Human Brain snRNA-seq Datasets
  • 05:57Conclusion

Summary

Automatic Translation

Intra-tumoral heterogeneity is an inherent feature of tumors, including gliomas. We developed a simple and efficient protocol that utilizes a combination of buffers and gradient centrifugation to isolate single nuclei from fresh frozen glioma tissues for single nucleus RNA and ATAC sequencing studies.

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