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JoVE Journal Cancer Research
Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
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Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Article DOI: 10.3791/61557-v • 09:22 min • February 28th, 2021
February 28th, 2021 •
1,2, 1,2, 1,2, 1,2, 1,2
1Department of Biomedical Sciences, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, 2Stem Cell Immunomodulation Research Center, University of Ulsan College of Medicine

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Summary
Automatic Translation

People with BRCA1 mutations have a higher risk of developing cancer, which warrants accurate evaluation of the function of BRCA1 variants. Herein, we described a protocol for functional assessment of BRCA1 variants using CRISPR-mediated cytosine base editors that enable targeted C:G to T:A conversion in living cells.

Tags

CRISPR-mediated Base Editors BRCA1 Variants Functional Assessment Disease Prevention Diagnosis Point Mutations Endogenously Expressed BRCA1 Exogenously Expressed BRCA1 Loss Of Function Mutations Cancer Development Drug Targets Essential Genes Viability Reduction BRCA1 Genome Sequence GenBank Target Sites Protospacer Adjacent Motif Sequence (PAM) Guide RNA Target Sequences Oligonucleotides
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