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Immunology and Infection

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JoVE Journal Immunology and Infection
Flow Cytometric Characterization of Murine B Cell Development
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Flow Cytometric Characterization of Murine B Cell Development

Article DOI: 10.3791/61565-v • 08:25 min • January 22nd, 2021
January 22nd, 2021 •
*1, *1, 1, 2, 1, 1, 1, 1, 3, 1
1Regeneron Tech Centers, Regeneron Pharmaceuticals, Inc., 2Regeneron Therapeutic Proteins, Regeneron Pharmaceuticals, Inc., 3Immunology and Inflammation, Regeneron Pharmaceuticals, Inc.
* These authors contributed equally

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Summary
Automatic Translation

We describe herein a simple analysis of the heterogeneity of the murine immune B cell compartment in the peritoneum, spleen, and bone marrow tissues by flow cytometry. The protocol can be adapted and extended to other mouse tissues.

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Flow Cytometric Characterization Murine B Cell Development Autoimmune Prone Mouse Models Humanized Mice Antibody Therapeutics Reagents Fluorophores Simultaneous Analysis B Cell Heterogeneity Phenotyping Genetically Engineered Mice Procedure Aliquoting Cells 96-well U-bottom Plate Samples And Controls Full Stain Sample Fluorescence-minus-one Samples Unstained Samples Bone Marrow Maturation Panel Spleen Maturation Panel Single Color Compensation Viability Controls Centrifuge Wash Cells
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