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Quantificazione del DNA circolante specifico del maiale nel sangue di un modello di xenotrapianto
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Quantificazione del DNA circolante specifico del maiale nel sangue di un modello di xenotrapianto

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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In questo protocollo sono stati progettati primer specifici suiini, sono stati costruiti frammenti di DNA specifici contenenti plasmidi e sono state stabilite curve standard per la quantificazione. Utilizzando primer specifici per specie, cpsDNA è stato quantificato da qPCR in modelli di trapianto di cellule da maiale a topo e modelli di trapianto di patch per arteria da maiale a scimmia.

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Biologia Numero 163 primer specifici DNA specifico del maiale circolante qPCR xenotrapianto biomarcatore rigetto immunitario
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