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Journal / Biology / Количественная оценка циркулирующей ДНК свиней в…
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Количественная оценка циркулирующей ДНК свиней в крови модели ксенотрансплантации
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Количественная оценка циркулирующей ДНК свиней в крови модели ксенотрансплантации

Article DOI: 10.3791/61579-v • 07:34 min • September 22nd, 2020
September 22nd, 2020 •
*1,2, *1,3, 1, 1, 4, 1,5, 6, 7, 1
1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Xianning Hospital of Traditional Chinese Medicine, 3Liver-biotechnology (Shenzhen) Co., Ltd., 4Department of Radiology, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital, 5Department of Life Sciences, University of Toronto, 6Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, 7Department of Hepatopancreatobiliary Surgery, Shenzhen University Health Science Center, Shenzhen University School of Medicine, First Affiliated Hospital of Shenzhen University, Shenzhen Second People's Hospital
* These authors contributed equally

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В этом протоколе были разработаны свиные специфические грунтовки, построены плазмиды, содержащие свиные фрагменты ДНК, и установлены стандартные кривые для количественной оценки. Используя вид-специфические грунтовки, cpsDNA было количественно qPCR в моделях трансплантации клетки свиньи к мыши и свинья-к-обезьяна модели трансплантации артерии.

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Биология Выпуск 163 специфические грунтовки циркулирующие свиньи конкретных ДНК qPCR ксенотрансплантация биомаркер иммунный отказ
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