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Biochemistry

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JoVE Journal Biochemistry
Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes
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Enabling Real-Time Compensation in Fast Photochemical Oxidations of Proteins for the Determination of Protein Topography Changes

Article DOI: 10.3791/61580-v • 07:38 min • September 1st, 2020
September 1st, 2020 •
1, 1,2,3
1Department of Biomolecular Sciences, University of Mississippi, 2Department of Chemistry and Biochemistry, University of Mississippi, 3GenNext Technologies, Inc.

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Summary
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Fast photochemical oxidation of proteins is an emerging technique for the structural characterization of proteins. Different solvent additives and ligands have varied hydroxyl radical scavenging properties. To compare the protein structure in different conditions, real-time compensation of hydroxyl radicals generated in the reaction is required to normalize reaction conditions.

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Real-time Compensation Fast Photochemical Oxidations Of Proteins Protein Topography Changes Protein Confirmation Protein Interactions Buffer Composition Sample Purity Protein Concentration Structural Problems Dosimetry Method Labeling Reactions Drug Development FPOP Optical Bench Laser Energy Per Pulse Polyimide Coding Absorbent Signal Inline Dosimeter Methanol
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