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Isolering af adipogene og fibroinflammatoriske stromacelleunderpopulationer fra murinerede intraabdominale fedtdepoter
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation of Adipogenic and Fibro-Inflammatory Stromal Cell Subpopulations from Murine Intra-Abdominal Adipose Depots
DOI:

06:50 min

August 16, 2020

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Chapters

  • 00:00Introduction
  • 00:35Adipocyte Precursor Cell (APC) and Fibro‐Inflammatory Progenitor (FIP) FACS Isolation
  • 02:22Adipogenic and Non‐Adipogenic Cell Fraction Immunomagnetic Bead Separation
  • 04:34Cell Culture and Differentiation
  • 05:07Results: Isolation and Differentiation of Adipogenic Stromal Cell Subset
  • 06:11Conclusion

Summary

Automatic Translation

Denne protokol beskriver den tekniske tilgang til at isolere adipogene og fibroinflammatoriske stromale cellesubpopulationer fra murine intra-abdominale hvide fedtvævsdepoter (WAT) ved fluorescensaktiveret cellesortering eller immunmagnetisk perleseparation.

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