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Journal / Biochemistry / En model membran platform for rekonstituerende…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
En model membran platform for rekonstituerende Mitokondrielle Membran Dynamics
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En model membran platform for rekonstituerende Mitokondrielle Membran Dynamics

Article DOI: 10.3791/61620-v • 10:31 min • September 2nd, 2020
September 2nd, 2020 •
1, 1, 2, 1,3
1Department of Molecular Biology, Massachusetts General Hospital, 2Department of Chemistry, University of Akron, 3Department of Genetics, Harvard Medical School

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Mitokondriel fusion er en vigtig hjemostatisk reaktion underliggende mitokondrielle dynamik. Beskrevet her er en in vitro rekonstitution system til at studere mitokondrielle indre membran fusion, der kan løse membran tøjring, docking, hemifusion, og pore åbning. Alsidigheden i denne tilgang til at udforske cellemembransystemer diskuteres.

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Denne måned i JoVE mitokondrielle indre membran Opa1 polymer-tøjret lipid bilayer in vitro rekonstitution membran fusion TIRF mikroskopi
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