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JoVE Journal Biology
Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates
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Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates

Article DOI: 10.3791/61623-v • 10:18 min • July 9th, 2020
July 9th, 2020 •
*1,2, *1,2, 1,2
1Cell Biology and Anatomy, Chicago Medical School, Rosalind Franklin University of Medicine and Science, 2Center for Brain Function and Repair, Rosalind Franklin University of Medicine and Science
* These authors contributed equally

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Summary
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This protocol presents a method for imaging neuronal population activity with single-cell resolution in non-transgenic invertebrate species using absorbance voltage-sensitive dyes and a photodiode array. This approach enables a rapid workflow, wherein imaging and analysis can be pursued over the course of a single day.

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Photodiode-based Optical Imaging Network Dynamics Single-neuron Resolution Non-transgenic Invertebrates Photodiode Array Voltage-sensitive Dyes Action Potentials Calcium Imaging Sharp Electrode Recordings Optical Imaging Workflow Visualization Of Network Activity Single Action Potential Resolution Non-transgenic Invertebrate Systems Vertebrate Systems Evan Hill Laboratory Protocol Aplysia Californica Anesthetizing Dissection Scissors Midline Incision Foot Flaps Central Nervous System Buccal Mass Cerebral Ganglia Nerve Stimulation
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