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JoVE Journal Immunology and Infection
Visualisation de Pseudomonas aeruginosa dans les expectorations de patients atteints de fibrose kystique
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Visualisation de Pseudomonas aeruginosa dans les expectorations de patients atteints de fibrose kystique

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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Ce protocole fournit des méthodes de visualisation des cellules bactériennes et du polysaccharide du locus de synthèse des polysaccharides (Psl) dans les expectorations des patients atteints de fibrose kystique.

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Immunologie et infection Numéro 161 Infection chronique Fonction pulmonaire Morbidité Antibiothérapie Patients pédiatriques Organisation spatiale Agrégation Formation de biofilm Production de polysaccharides Échantillons d’expectorations Technique de nettoyage des tissus Matrice d’hydrogel Structures 3D Marqueurs fluorescents Hybridation in situ par fluorescence Anticorps anti-PSL Exopolysaccharide Coloration DAPI Cellules hôtes Imagerie haute résolution
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