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Journal / Immunology and Infection / 嚢胞性線維症患者の喀痰中の 緑膿菌…
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Immunology and Infection

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JoVE Journal Immunology and Infection
嚢胞性線維症患者の喀痰中の 緑膿菌 の可視化
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嚢胞性線維症患者の喀痰中の 緑膿菌 の可視化

Article DOI: 10.3791/61631-v • 07:25 min • July 16th, 2020
July 16th, 2020 •
1, 2, 1, 1, 1,3, 1,4
1Translational Medicine, Hospital for Sick Children, 2Center for Microbial Pathogenesis, Department of Pediatrics, University of Pittsburgh, 3Microbiology, Department of Pediatric Laboratory Medicine, Hospital for Sick Children, 4Infectious Diseases, Department of Pediatrics, Hospital for Sick Children

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このプロトコルは嚢胞性線維症の患者の喀痰内の細菌のセルそして多糖類の統合の遺伝子座(Psl)の多糖類の視覚化のための方法を提供する。

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免疫学と感染症、第161号、慢性感染症、肺機能、罹患率、抗生物質療法、小児患者、空間構成、凝集、バイオフィルム形成、多糖類産生、喀痰サンプル、組織透明化技術、ハイドロゲルマトリックス、3D構造、蛍光標識、蛍光In Situハイブリダイゼーション、抗PSL抗体、エキソ多糖、DAPI染色、宿主細胞、高解像度イメージング
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