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Explorando estrutura tecidual adiposa por limpeza de metilsalicylate e imagem 3D
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Explorando estrutura tecidual adiposa por limpeza de metilsalicylate e imagem 3D

Article DOI: 10.3791/61640-v • 10:10 min • August 19th, 2020
August 19th, 2020 •
1, 1, 2, 3, 1, 4, 4, 1, 1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France

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Aqui, descrevemos um método simples, barato e rápido de limpeza para resolver a estrutura 3D do tecido adiposo branco e camundongo usando uma combinação de marcadores para visualizar vasculatura, núcleos, células imunes, neurônios e proteínas de casaco de gotícula lipídica por imagem fluorescente.

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Biologia Edição 162 Tecido adiposo limpeza microscopia leve tecido inteiro 3D obesidade morfologia
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