Journal
/
Biology
/
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

Dosimetry for Cell Irradiation using Orthovoltage (40-300 kV) X-Ray Facilities

DOI:
10.3791/61645-v

•

06:51 min

•

February 20, 2021

•

, , , ,
1Department of RAdiobiology and Regenerative MEDicine (SERAMED), Laboratory of Radiobiology of Accidental Exposures (LRAcc),Institute for Radiological Protection and Nuclear Safety (IRSN), 2Department of RAdiobiology and Regenerative MEDicine (SERAMED), Laboratory of MEDical Radiobialogy (LRMed),Institute for Radiological Protection and Nuclear Safety (IRSN), 3Department of DOSimetry (SDOS), Ionizing Radiation Dosimetry Laboratory (LDRI),Institute for Radiological Protection and Nuclear Safety (IRSN)

Chapters

  • 00:05Introduction
  • 00:44Irradiation Field Evaluation (No Dose Estimation)
  • 01:26Dose Rate Measurement
  • 02:08Cell Culture Medium Attenuation and Scattering Measurement
  • 03:28Self-Developing Dosimetry Radiochromic Film Analysis
  • 04:37Results: Representative Irradiation Analyses
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

This document describes a new dosimetry protocol for cell irradiations using low energy X-ray equipment. Measurements are performed in conditions simulating real cell irradiation conditions as much as possible.

Tags

Keywords: DosimetryCell IrradiationOrthovoltageX-ray FacilitiesRadiobiological StudiesDose MeasurementIonization ChamberDose RateSelf-developing Dosimetry FilmCalibration CurveCell Culture Medium AttenuationScattering

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61645?article_type=v

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography
check_url/61645?article_type=v

Protein Crystallization for X-ray Crystallography
check_url/61645?article_type=v

Planarian Immobilization, Partial Irradiation, and Tissue Transplantation
check_url/61645?article_type=v

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development
check_url/61645?article_type=v

Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes
check_url/61645?article_type=v

3D Printing of Preclinical X-ray Computed Tomographic Data Sets
check_url/61645?article_type=v

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
check_url/61645?article_type=v

Production of Human Norovirus Protruding Domains in E. coli for X-ray Crystallography
check_url/61645?article_type=v

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
check_url/61645?article_type=v

Micron-scale Phenotyping Techniques of Maize Vascular Bundles Based on X-ray Microcomputed Tomography

Read Article