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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolamento e Identificação Conjuntivival Commensal em Camundongos
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Isolamento e Identificação Conjuntivival Commensal em Camundongos

Article DOI: 10.3791/61672-v • 07:52 min • May 1st, 2021
May 1st, 2021 •
1, 1, 1, 2,3, 2,3, 1
1Department of Medicine, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School

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Apresentado aqui é um protocolo para o isolamento e amplificação de bactérias comensais anaeróbicas aeróbicas e facultativas de camundongos anastrérmicos utilizando um cotonete de olho único e passo de enriquecimento baseado em cultura com identificação subsequente por métodos à base microbiológica e espectrometria de massa MALDI-TOF.

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Este mês em JoVE Edição 171 microbioma commensal do mouse ou conjuntiva cotonete de olho único amplificação comensal anaeróbica aeróbica/facultativa classificação microbiológica tempo de deserção a laser assistido matricial de identificação de espectrofotometria de massa de voo identificação MALDI-TOF relativa abundância commensal
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