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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis
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An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

Article DOI: 10.3791/61677-v • 06:10 min • August 6th, 2020
August 6th, 2020 •
1, 2, 1, 1, 1,3, 1
1Department of Ophthalmology, Boston Children's Hospital, Harvard Medical School, 2Department of Clinical and Metabolic Genetics, Hospital for Sick Children, University of Toronto, 3Manton Center for Orphan Disease, Harvard Medical School, Boston Children's Hospital

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This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue.

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Ex Vivo Choroid Sprouting Assay Ocular Microvascular Angiogenesis Choroidal Angiogenesis Research Age Related Macular Degeneration (AMD) Neovascular AMD Treatment Choroidal Neovascularization Wild Type Mouse Tissue Genetically Modified Mouse Tissue Penicillin Streptomycin Commercially Available Supplements Complete Classic Medium With Serum Basal Membrane Extract (BME) Dissecting Microscope Forceps Scissors 70% Ethanol Cell Culture Dishes Corneal Limbus Cornea Iris Complex Vitreous Lens
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