5,614 Views
•
06:10 min
•
August 06, 2020
DOI:
Summary
Automatically generated
This protocol presents choroid sprouting assay, an ex vivo model of microvascular proliferation. This assay can be used to assess pathways involved in proliferating choroidal micro vessels and assess drug treatments using wild type and genetically modified mouse tissue.
Explore More Videos
Related Videos
06:13
A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
Related Videos
16024 Views
06:54
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
Related Videos
6857 Views
08:47
Whole Mount Immunofluorescent Staining of the Neonatal Mouse Retina to Investigate Angiogenesis In vivo
Related Videos
45791 Views
11:49
The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
Related Videos
16756 Views
10:47
Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro
Related Videos
30437 Views
06:51
Retinal Explant of the Adult Mouse Retina as an Ex Vivo Model for Studying Retinal Neurovascular Diseases
Related Videos
4068 Views
09:16
Investigating Angiogenesis on a Functional and Molecular Level by Leveraging the Scratch Wound Migration Assay and the Spheroid Sprouting Assay
Related Videos
1185 Views
08:04
In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing
Related Videos
2841 Views
10:00
2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue
Related Videos
252 Views
01:58
Choroid Sprouting Assay to Study Ocular Microvascular Proliferation Ex Vivo
Related Videos
221 Views
Read Article
Cite this Article
Tomita, Y., Shao, Z., Cakir, B., Kotoda, Y., Fu, Z., Smith, L. E. An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis. J. Vis. Exp. (162), e61677, doi:10.3791/61677 (2020).
Copy