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Journal / Cancer Research / Isolering av proximala vätskor för att undersöka…
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Cancer Research

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JoVE Journal Cancer Research
Isolering av proximala vätskor för att undersöka tumörmikromiljön i bukspottskörtelns adenokarcinom
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Isolering av proximala vätskor för att undersöka tumörmikromiljön i bukspottskörtelns adenokarcinom

Article DOI: 10.3791/61687-v • 05:44 min • November 5th, 2020
November 5th, 2020 •
*1, *2, 1,3, 1,3, 4,5,6, 1,3, 2,7, 2
1Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences, Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 7Department of Medical Biotechnology and Translational Medicine, University of Milan
* These authors contributed equally

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Bukspottkörteljuice är en värdefull källa till biomarkörer för human bukspottkörtelcancer. Vi beskriver här en metod för intraoperativ insamlingsprocedur. För att övervinna utmaningen att anta denna procedur i murina modeller föreslår vi ett alternativt prov, tumörinterstitiell vätska, och beskriver här två protokoll för dess isolering.

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Cancerforskning Utgåva 165 pankreas adenokarcinom bukspottkörteljuice tumörinterstitiell vätska proximal vätska flytande biopsi biomarkörer proteomik
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