Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

Jing Zhang; Jing Huang; Ishani Majumdar; Ryan C. James; Julia Gichimu; Manikandan Paramasivam; Durga Pokharel; Himabindu Gali; Marina  A. Bellani; Michael  M Seidman

doi:10.3791/61689

Journal

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Biology

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Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

JoVE Journal
Biology

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JoVE Journal Biology

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

1,361 Views

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08:24 min

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July 27, 2021

DOI:

10.3791/61689-v

DOI:

10.3791/61689-v

•

08:24 min

July 27, 2021

•

1351 Views

Jing Zhang*¹, Jing Huang*², Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging,National Institutes of Health ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology,Hunan University ³Department of Molecular Biology and Genetics,Cornell University ⁴Department of Cellular and Molecular Medicine,University of Copenhagen ⁵Horizon Discovery ⁶Boston University School of Medicine

Summary

While replication fork collisions with DNA adducts can induce double strand breaks, less is known about the interaction between replisomes and blocking lesions. We have employed the proximity ligation assay to visualize these encounters and to characterize the consequences for replisome composition.