Journal
/
/
In Vitro evaluering af onkogene transformation i humane mammary epitelceller
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
DOI:

09:44 min

September 24, 2020

, , , ,

Chapters

  • 00:04Introduction
  • 00:42Breast Primary Epithelial Cell Passaging and Population Doubling Calculation
  • 02:12Three-Dimensional Cell Culture in Basement Membrane Matrix and Immunofluorescent Protein Detection
  • 05:07Anchorage-Independent Assay and Colony Quantification
  • 07:19Results: Representative BPEC Transformation Evaluation
  • 09:15Conclusion

Summary

Automatic Translation

Denne protokol giver eksperimentelle in vitro værktøjer til at evaluere omdannelsen af menneskelige mælkeceller. Detaljerede trin til opfølgende celleproliferedningshastighed, forankrings-uafhængig vækstkapacitet og fordeling af cellestælg i 3D-kulturer med kældermembranmatrix er beskrevet.

Related Videos

Read Article