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In Vitro evaluering af onkogene transformation i humane mammary epitelceller
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1Department of Cell Biology, Physiology and Immunology,Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities,IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology,IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell),IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine,University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences,University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology,University of Vic
Chapters
- 00:04Introduction
- 00:42Breast Primary Epithelial Cell Passaging and Population Doubling Calculation
- 02:12Three-Dimensional Cell Culture in Basement Membrane Matrix and Immunofluorescent Protein Detection
- 05:07Anchorage-Independent Assay and Colony Quantification
- 07:19Results: Representative BPEC Transformation Evaluation
- 09:15Conclusion
Denne protokol giver eksperimentelle in vitro værktøjer til at evaluere omdannelsen af menneskelige mælkeceller. Detaljerede trin til opfølgende celleproliferedningshastighed, forankrings-uafhængig vækstkapacitet og fordeling af cellestælg i 3D-kulturer med kældermembranmatrix er beskrevet.
