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In Vitro Evaluatie van Oncogene Transformatie in Human Mammary Epithelial Cells
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vitro Evaluation of Oncogenic Transformation in Human Mammary Epithelial Cells
DOI:

09:44 min

September 24, 2020

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Chapters

  • 00:04Introduction
  • 00:42Breast Primary Epithelial Cell Passaging and Population Doubling Calculation
  • 02:12Three-Dimensional Cell Culture in Basement Membrane Matrix and Immunofluorescent Protein Detection
  • 05:07Anchorage-Independent Assay and Colony Quantification
  • 07:19Results: Representative BPEC Transformation Evaluation
  • 09:15Conclusion

Summary

Automatic Translation

Dit protocol biedt experimentele in vitro tools om de transformatie van menselijke borstcellen te evalueren. Gedetailleerde stappen naar de follow-up cel proliferatie tarief, verankering-onafhankelijke groeicapaciteit, en de distributie van cellijnen in 3D-culturen met kelder membraan matrix worden beschreven.

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