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In Vitro evaluering av onkogen transformasjon i humane pattedyr epitelceller
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In Vitro evaluering av onkogen transformasjon i humane pattedyr epitelceller

Article DOI: 10.3791/61716-v • 09:44 min • September 24th, 2020
September 24th, 2020 •
1,2, 1,3,4, 5,6,7, 1, 1
1Department of Cell Biology, Physiology and Immunology, Universitat Autònoma de Barcelona, 2Optical Microscopy Core Facilities, IDIBELL, 3Hereditary Cancer Program, Catalan Institute of Oncology, IDIBELL, 4Program in Molecular Mechanisms and Experimental Therapy in Oncology (Oncobell), IDIBELL, 5New Therapeutic Strategies in Cancer Group. Department of Biochemistry and Molecular Biomedicine, School of Biology, Institute of Biomedicine, University of Barcelona (IBUB), 6Department of Biochemistry & Physiology, School of Pharmacy and Food Sciences, University of Barcelona, 7Department of Biosciences, Faculty of Sciences and Technology, University of Vic

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Denne protokollen gir eksperimentelle in vitro verktøy for å evaluere transformasjonen av menneskelige brystceller. Detaljerte trinn for oppfølging av cellespredningshastighet, forankringsuavhengig vekstkapasitet og distribusjon av cellelinje i 3D-kulturer med kjellermembranmatrise er beskrevet.

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Kreftforskning Utgave 163 cellulær transformasjon vekstkurve forankringsanalyse 3D-cellekultur humane pattedyrepitelceller tumorgenese
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