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Journal / Biochemistry / MS2-Affinity Purification Coupled with RNA…
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Biochemistry

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JoVE Journal Biochemistry
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Article DOI: 10.3791/61731-v • 08:34 min • February 23rd, 2021
February 23rd, 2021 •
1, 2, 2, 1, 1, 1
1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke

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Summary
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MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria. 

Tags

MS2-affinity Purification RNA Sequencing MAPS Gram-positive Bacteria SRNA Partners Regulatory Networks Virulence Factors Production Biofilm Formation Staphylococcal Infections Pathogenic Bacteria Non-pathogenic Bacteria Noemie Mercier Pascal Romby's Laboratory BHI Medium Erythromycin OD 600 Centrifuge Tube Mechanical Cell Lysis
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