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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells
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Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

Isolation, Proliferation and Differentiation of Rhesus Macaque Adipose-Derived Stem Cells

DOI:
10.3791/61732-v

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07:33 min

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May 26, 2021

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, ,
1Department of Physiology,Louisiana State University Health Sciences Center – New Orleans, 2Comprehensive Alcohol-HIV/AIDS Research Center,Louisiana State University Health Sciences Center – New Orleans

Chapters

  • 00:04Introduction
  • 00:34Isolation of Adipose-Derived Stem Cells-ADSC
  • 04:02ADSC Proliferation
  • 05:23Adipogenic Differentiation
  • 06:01Results: Observation of ADSCs Using Light Microscopy
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

In this article, we describe isolation of rhesus macaque derived adipose-derived stem cells (ADSCs) using an enzymatic tissue digestion protocol. Next, we describe ADSC proliferation which includes cell detachment, counting and plating. Lastly, ADSC differentiation is described using specific adipogenic inducing agents. Additionally, we describe staining techniques to confirm differentiation.

Tags

Adipose-derived Stem CellsRhesus MacaqueIsolationProliferationDifferentiationMetabolic AssaysInsulin-stimulated Glucose UptakeLipogenesisLipolysisCollagenase DigestionRBC LysisCell Culture

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