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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
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Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat

DOI:
10.3791/61750-v

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09:49 min

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June 09, 2021

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, , ,
1Keenan Research Center for Biomedical Science,St Michaels Hospital, Unity Health Toronto, 2Department of Medicine and Interdepartmental Division of Critical Care Medicine,University of Toronto

Chapters

  • 00:04Introduction
  • 01:03Muscle Digestion
  • 02:28Generation of Single Cell Suspension
  • 04:06Antibody Staining for Flow Cytometry
  • 06:52Results: Identification of FAPs and MPs via Flow Cytometry
  • 09:20Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines a method to isolate Fibro-adipogenic progenitors (FAPs) and myogenic progenitors (MPs) from rat skeletal muscle. Utilization of the rat in muscle injury models provides increased tissue availability from atrophic muscle for the analysis and a larger repertoire of validated methods to assess muscle strength and gait in free-moving animals.

Tags

Keywords: Fibro-Adipogenic Progenitors (FAPs)Myogenic Progenitors (MPs)Skeletal MuscleRatMuscle RegenerationFibrosisCollagenase IIDispaseCell Strainer

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