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Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

DOI:
10.3791/61757-v

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09:33 min

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September 11, 2020

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, , , ,
1Research & Development,Postnova Analytics GmbH

Chapters

  • 00:05Introduction
  • 00:49Asymmetrical Flow Field-Flow Fractionation (AF4)-UV-Visible Spectroscopy System Preparation and Qualification
  • 02:28AF4-UV-vis Sample Analysis
  • 04:22Data Evaluation
  • 07:16Results: Representative AF4 Gold Nanoparticle Sizing
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the use of Asymmetrical Flow Field-Flow Fractionation coupled with UV-vis detection for the determination of the size of an unknown gold nanoparticle sample.

Tags

Keywords: Asymmetrical Flow Field-flow FractionationAF4Gold NanoparticlesSize DistributionSuspensionExternal Size CalibrationNanoparticle Size StandardsUV-vis DetectionSample PreparationSystem QualificationFractionation Method Parameters

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