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Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana
 

Production of IgG Fusion Proteins Transiently Expressed in Nicotiana benthamiana

Article doi: 10.3791/61774
January 16th, 2021

Summary January 16th, 2021

We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana benthamiana. This protocol can be extended to purification and visualization of numerous proteins that utilize column chromatography. Moreover, the protocol is adaptable to the in-person and virtual college teaching laboratory, providing project-based exploration.

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