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Measuring Volatile and Non-volatile Antifungal Activity of Biocontrol Products
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Chapters
- 00:04Introduction
- 00:55Conidia Recovery
- 01:28Fungal Plate Preparation
- 02:13Garlic Contact-Inhibition Plate Preparation
- 03:00Contact-Inhibition Assay
- 03:47Vapor-Phase Antifungal Inhibition Assay
- 04:56Results: Representative Antifungal Compound Inhibition of Spore, Early Hyphae, and Mycelium Growth
- 06:20Conclusion
We describe a modified agar-based method designed to quantify the antifungal effects of plant-derived products. Both volatile and non-volatile contributions to the antifungal activity can be assessed through this protocol. In addition, efficacy against fungi can be measured at key developmental stages in a single experimental setup.
Tags
Keyword Extraction:
Volatile Antifungal ActivityNon-volatile Antifungal ActivityBiocontrol ProductsFungal Growth StagesPlant-derived ProductsAntifungal CompoundsMode Of ApplicationBiocontrolConidia CollectionTrichoderma Mycelium CultureConidia SolutionFungal PlatesPDA MediumGlass BeadsContact-inhibition AssayGarlic PowderPDA AgarAntifungal Compound-treated Dishes
