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Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
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Biochemistry
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JoVE Journal Biochemistry
Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

DOI:
10.3791/61799-v

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09:09 min

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October 13, 2020

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, , ,
1Department of Molecular and Cellular Biology,University of Guelph, 2Mass Spectrometry Facility,University of Guelph

Chapters

  • 00:05Introduction
  • 01:07Peptidoglycan Sacculi Extraction
  • 03:17Liquid Chromatography-Mass Spectrometry (LC-MS) Chromatogram Spectral Processing
  • 04:11Differential Spectral Feature Analysis
  • 05:06Muropeptide Annotation Spectral Features
  • 06:18Muropeptide Global Change Modification Assessment
  • 07:07Results: Representative Muropeptide Fragmentation and Identification and Peptidoglycan Composition Analysis
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol covers a detailed analysis of peptidoglycan composition using liquid chromatography mass spectrometry coupled with advanced feature extraction and bioinformatic analysis software.

Tags

PeptidoglycanMass SpectrometryBioinformaticsPeptidoglycomicsSacculi ExtractionSDS TreatmentEnzymatic DigestionLiquid Chromatography-mass Spectrometry

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