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Journal / Cancer Research / Twee flow cytometrische benaderingen van NKG2D…
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Cancer Research

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JoVE Journal Cancer Research
Twee flow cytometrische benaderingen van NKG2D Ligand-oppervlaktedetectie om stamcellen te onderscheiden van bulksubpopulaties bij acute myeloïde leukemie
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Twee flow cytometrische benaderingen van NKG2D Ligand-oppervlaktedetectie om stamcellen te onderscheiden van bulksubpopulaties bij acute myeloïde leukemie

Article DOI: 10.3791/61803-v • 05:24 min • February 21st, 2021
February 21st, 2021 •
*1, *1, 2, 1, 3, 1, 1,2,3
1Department of Biomedicine, University Hospital Basel and University of Basel, 2Division for Hematology, University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen
* These authors contributed equally

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We presenteren twee verschillende kleuringsprotocollen voor NKG2D ligand (NKG2DL) detectie in menselijke primaire acute myeloïde leukemie (AML) monsters. De eerste benadering is gebaseerd op een fusie-eiwit, in staat om alle bekende en potentieel nog onbekende liganden te herkennen, terwijl het tweede protocol afhankelijk is van de toevoeging van meerdere anti-NKG2DL-antilichamen.

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Kankeronderzoek NKG2DL CD34 AML LSC fusie-eiwit FACS
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