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Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model
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Cancer Research
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JoVE Journal Cancer Research
Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

DOI:
10.3791/61805-v

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08:32 min

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October 02, 2020

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, , , , , ,
1Department of Biomedical Sciences and Pathobiology,Virginia-Maryland College of Veterinary Medicine, 2Graduate Program in Translational Biology, Medicine and Health,Virginia Polytechnic Institute and State University, 3Department of Biomedical Engineering and Mechanics,Virginia Polytechnic Institute and State University, 4Department of Basic Science Education, Virginia Tech Carilion School of Medicine,Virginia Polytechnic Institute and State University

Chapters

  • 00:04Introduction
  • 00:51Processing Tissues
  • 02:49Staining Plates
  • 04:01Image Analysis
  • 06:26Results: Fiji-ImageJ Analysis is Accurate and Precise in Determining Metastatic Burden
  • 08:04Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ.

Tags

Keywords: Computer-based Image AnalysisLung Metastasis4T1 Breast Cancer ModelQuantificationPrecisionEfficiencyBreast Cancer TherapiesCollagenaseElastaseCell Strainer6-thioguanineMethanol Fixation

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