Journal
/
Biochemistry
/
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

DOI:
10.3791/61823-v

•

05:56 min

•

November 12, 2020

•

, , , , , , ,
1CNRS, Inserm, Centre d’Immunologie de Marseille-Luminy,Aix Marseille Univ, 2Faculty of Biotechnology,University of Wroclaw, 3Faculty of Biological Sciences,University of Wroclaw, 4CNRS, Centrale Marseille, Institut Fresnel,Aix Marseille Univ

Chapters

  • 00:05Introduction
  • 00:36Daily Checkpoint
  • 01:31Spot Size Calibration
  • 02:36svFCS Data Acquisition
  • 03:56Results: Representative svFCS Diffusion Law and Total Cholesterol Comparison
  • 05:07Conclusion

Summary

Automatic Translation

Automatic Translation

This article aims to present a protocol on how to build a spot variation Fluorescence Correlation Spectroscopy (svFCS) microscope to measure molecular diffusion at the plasma membrane of living cells.

Tags

Keywords: Spot Variation Fluorescence Correlation SpectroscopyMolecular DiffusionPlasma MembraneLiving CellsConfocal MicroscopyLaser PowerRhodamine 6GAuto Correlation FunctionDiffusion TimeSvFCS AnalysisXY ScanZ Scan

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61823?article_type=v

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
check_url/61823?article_type=v

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
check_url/61823?article_type=v

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
check_url/61823?article_type=v

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes
check_url/61823?article_type=v

Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
check_url/61823?article_type=v

Analysis of Thylakoid Membrane Protein Complexes by Blue Native Gel Electrophoresis
check_url/61823?article_type=v

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
check_url/61823?article_type=v

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
check_url/61823?article_type=v

Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
check_url/61823?article_type=v

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Read Article