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JoVE Journal Cancer Research
Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Article DOI: 10.3791/61830-v • 06:54 min • October 27th, 2020
October 27th, 2020 •
1, 1, 1, 1, 1
1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

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Summary
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We describe a systematic workflow to investigate TGF-β signaling and TGF-β-induced EMT by studying the protein and gene expression involved in this signaling pathway. The methods include Western blotting, a luciferase reporter assay, qPCR, and immunofluorescence staining.

Tags

TGF-beta Signaling EMT Breast Cancer Normal Cells Modulators SMAD Signaling NMuMG Cells E-cadherin Staining Filamentous-actin Organization Morphological Changes Mesenchymal Phenotype Cancer Progression Migration Invasion Metastasis Chemotherapy Resistance Cell Density Tissue Fibrosis
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