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Journal / Cancer Research / TGF-β Signalering en TGF-β-geïnduceerde…
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Cancer Research

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JoVE Journal Cancer Research
TGF-β Signalering en TGF-β-geïnduceerde Epitheel-naar-mesenchymale overgang bij borstkanker en normale cellen bestuderen
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TGF-β Signalering en TGF-β-geïnduceerde Epitheel-naar-mesenchymale overgang bij borstkanker en normale cellen bestuderen

Article DOI: 10.3791/61830-v • 06:54 min • October 27th, 2020
October 27th, 2020 •
1, 1, 1, 1, 1
1Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

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We beschrijven een systematische workflow om TGF-β signalering en TGF-β-geïnduceerde EMT te onderzoeken door de eiwit- en genexpressie te bestuderen die betrokken zijn bij deze signaleringsroute. De methoden omvatten western blotting, een luciferase reporter assay, qPCR, en immunofluorescentie vlekken.

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Cancer Research transforming growth factor-β epithelial-to-mesenchymal transition borstkanker Western blotting transcriptional reporter immunofluorescentie inhibitors adipogenesis
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