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Biology

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Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito
 

Studying the Activity of Neuropeptides and Other Regulators of the Excretory System in the Adult Mosquito

Article DOI: 10.3791/61849-v
August 24th, 2021

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Summary August 24th, 2021

This protocol outlines methodologies behind the Ramsay assay, ion-selective microelectrodes, Scanning Ion-selective Electrode Technique (SIET) and in vitro contraction assays, applied to study the adult mosquito excretory system, comprised of the Malpighian tubules and hindgut, to collectively measure ion and fluid secretion rates, contractile activity, and transepithelial ion transport.

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