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JoVE Journal
Biology

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An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice
 

An Antegrade Perfusion Method for Cardiomyocyte Isolation from Mice

Article doi: 10.3791/61866
May 19th, 2021

Summary May 19th, 2021

We developed a simple method for isolating high quality individual mouse heart cells by the antegrade perfusion technique. This method is Langendorff-free and useful for isolating ventricular and atrial myocytes or interstitial cells, such as cardiac fibroblasts or progenitors.

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