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Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices
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JoVE Journal Bioengineering
Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices

Electric-Field-Induced Neural Precursor Cell Differentiation in Microfluidic Devices

DOI:
10.3791/61917-v

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07:15 min

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April 14, 2021

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, ,
1Research Center for Applied Sciences,Academia Sinica, 2Institute of Biophotonics,National Yang Ming Chao Tung University, 3Department of Mechanical and Mechatronic Engineering,National Taiwan Ocean University, 4College of Engineering,Chang Gung University

Chapters

  • 00:05Introduction
  • 00:46Design and Fabrication of the Multichannel, Optically Transparent, Electrotactic Chip and Coating Poly-L-lysine on the Substrate in the Cell Culture Regions
  • 02:49Coating Poly-L-lysine on the Substrate in the Cell Culture Regions and Preparation of the Salt Bridge Network
  • 04:47Setup of the Microfluidic System for DC Pulse Stimulation
  • 05:55Results: Differentiation of the mNPC Cells in the Control Group and in the DC Pulse Stimulation Group at DIV 3, 7, and 14
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system.

Tags

Keywords: Electric FieldNeural Precursor CellsDifferentiationMicrofluidic DevicesPMMALaser ScriberThermal BonderPLLCell CultureNeural Regeneration

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