00:46Design and Fabrication of the Multichannel, Optically Transparent, Electrotactic Chip and Coating Poly-L-lysine on the Substrate in the Cell Culture Regions
02:49Coating Poly-L-lysine on the Substrate in the Cell Culture Regions and Preparation of the Salt Bridge Network
04:47Setup of the Microfluidic System for DC Pulse Stimulation
05:55Results: Differentiation of the mNPC Cells in the Control Group and in the DC Pulse Stimulation Group at DIV 3, 7, and 14
In this study, we present a protocol for the differentiation of neural stem and progenitor cells (NPCs) solely induced by direct current (DC) pulse stimulation in a microfluidic system.