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In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti

Article DOI: 10.3791/61947-v • 08:08 min • December 4th, 2020
December 4th, 2020 •
*1,2,3, *1,2, 1,2, 1,2, 3
1Department of Otorhinolaryngology, Yonsei University Wonju College of Medicine, 2Research Institute of Hearing Enhancement, Yonsei University Wonju College of Medicine, 3Department of Otorhinolaryngology Head and Neck Surgery, Hallym University College of Medicine
* These authors contributed equally

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Summary
Automatic Translation

In this study, we present a real-time imaging method using confocal microscopy to observe cells moving toward damaged tissue by ex vivo incubation with the cochlear epithelium containing the organ of Corti.

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In Vitro Time-lapse Live-cell Imaging Cell Migration Organ Of Corti MSCs Transwell Wound Healing Protocols Explant Culture Homing Efficiency Tectorial Membrane Removal Sterilization Laminar Flow Tissue Culture Hood Dissection Instruments Decapitating Mouse
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