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Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
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Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay

DOI:
10.3791/61950-v

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05:17 min

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February 09, 2021

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, , , , ,
1Department of Biology and Chemistry,Azusa Pacific University

Chapters

  • 00:04Introduction
  • 00:36Cytotoxicity Assay Using MTT
  • 03:39Results: Effects of Test Compounds on COS-7 Cell Viability in a Modified MTT Assay
  • 04:50Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a bioassay using 3-(4′,5′-dimethylthiazol-2′-yl)-2,5- diphenyltetrazolium bromide (MTT) to test previously synthesized spirocyclic oximes.

Tags

Keywords: Spirocyclic CompoundsMTT AssayCytotoxicitySolid-phase SynthesisHeterocyclesCell ViabilityDMSOFormazan CrystalsAbsorbanceCOS7 CellsCisplatin

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