Journal
/
Bioengineering
/
Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology
JoVE Journal
Bioengineering
Author Produced
This content is Free Access.
JoVE Journal Bioengineering
Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

DOI:
10.3791/61962-v

•

10:41 min

•

January 23, 2021

•

,
1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research,Royal North Shore Hospital, 4University of Technology, Sydney

Chapters

  • 00:00Introduction
  • 00:54Cardiac Spheroid Formation
  • 05:46Drug Testing
  • 06:42Representative Results
  • 07:36Endothelial Cell Network Staining
  • 09:52Representative Results
  • 10:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.

Tags

Keywords: Cardiac SpheroidsIn VitroHuman Heart PathophysiologyCardiac MyocytesCardiac FibroblastsCardiac Endothelial CellsDrug Toxicity AssaysDoxorubicinCardiomyocytesCell CultureHanging DropSpheroid Formation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61962?article_type=v

Multiparametric Optical Mapping of the Langendorff-perfused Rabbit Heart
check_url/61962?article_type=v

Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
check_url/61962?article_type=v

Design of a Cyclic Pressure Bioreactor for the Ex Vivo Study of Aortic Heart Valves
check_url/61962?article_type=v

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
check_url/61962?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/61962?article_type=v

Construction of Defined Human Engineered Cardiac Tissues to Study Mechanisms of Cardiac Cell Therapy
check_url/61962?article_type=v

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
check_url/61962?article_type=v

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
check_url/61962?article_type=v

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
check_url/61962?article_type=v

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

Read Article