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Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Article DOI: 10.3791/61972-v • 10:45 min • March 20th, 2021
March 20th, 2021 •
1,4, 1,2, 3, 2, 1, 1, 1, 1, 2, 2, 1
1Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, 2Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, 3Paul Scherrer Institute, Swiss Light Source, 4European XFEL GmbH

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Summary
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A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.

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Crystallization Structural Determination Enzyme Substrate Complex Serial Crystallography Microfluidic Chip Laboratory Architecture Reactivity Of RNA Institute Of Molecular And Cell Biology Strasbourg Biomolecule X-ray Diffraction ChipX Counter-diffusion Crystal Handling Cryo-cooling Microscope Slide Microfluidic Channels Crystallization Chambers
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