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Journal / Genetics / Riprogrammazione diretta dei fibroblasti umani in …
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Genetics
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JoVE Journal Genetics
Riprogrammazione diretta dei fibroblasti umani in mioblasti per indagare le terapie per i disturbi neuromuscolari
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Riprogrammazione diretta dei fibroblasti umani in mioblasti per indagare le terapie per i disturbi neuromuscolari

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Questo protocollo descrive la conversione dei fibroblasti cutanei in mioblasti e la loro differenziazione in miotubi. Le linee cellulari sono derivate da pazienti con disturbi neuromuscolari e possono essere utilizzate per indagare meccanismi patologici e testare strategie terapeutiche.

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Genetica Numero 170 Riprogrammazione hTERT MioD fibroblasto mioblasto disturbo neuromuscolare test terapeutici
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