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Journal / Genetics / CRISPR/Cas9 Editing van het C. elegans rbm-3.2…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 Editing van het C. elegans rbm-3.2 Gen met behulp van de dpy-10 Co-CRISPR Screening Marker en Geassembleerde Ribonucleoproteïne Complexen.
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CRISPR/Cas9 Editing van het C. elegans rbm-3.2 Gen met behulp van de dpy-10 Co-CRISPR Screening Marker en Geassembleerde Ribonucleoproteïne Complexen.

Article DOI: 10.3791/62001-v • 07:46 min • December 11th, 2020
December 11th, 2020 •
1, *1, *1, *1, 1
1Department of Chemistry and Biochemistry, The University of Tulsa
* These authors contributed equally

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Het doel van dit protocol is om naadloze CRISPR/Cas9-bewerking van het C. elegans-genoom mogelijk te maken met behulp van geassembleerde ribonucleoproteïnecomplexen en de dpy-10 co-CRISPR-marker voor screening. Dit protocol kan worden gebruikt om een verscheidenheid aan genetische modificaties in C. elegans te maken, waaronder inserties, deleties, genvervangingen en codonsubstituties.

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Genetica CRISPR/Cas9 C. elegans ribonucleoproteïne complexen genoomtechnologie rbm-3.2 gene editing micro-injectie CRISPR screening
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