Journal
/
Biology
/
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

Gonadectomy and Blood Sampling Procedures in the Small Size Teleost Model Japanese Medaka (Oryzias latipes)

DOI:
10.3791/62006-v

•

05:31 min

•

December 11, 2020

•

, , , , , ,
1Physiology Unit, Faculty of Veterinary Medicine,Norwegian University of Life Sciences, 2Laboratory of Physiology, Atmosphere and Ocean Research Institute,The University of Tokyo, 3Department of Biological Sciences, Graduate School of Science,The University of Tokyo, 4Centre of Reproduction, Development and Aging (CRDA), Faculty of Health Sciences,University of Macau

Chapters

  • 00:04Introduction
  • 00:50Gonadectomy
  • 02:22Blood Sampling
  • 03:16Results: E2 and 11-KT Levels in Females and Males of Sham-Operated and Gonadectomized Fish
  • 04:54Conclusion

Summary

Automatic Translation

Automatic Translation

The article describes a quick protocol to gonadectomize and sample blood from the small teleost fish, using Japanese medaka (Oryzias latipes) as a model, to investigate the role of sex steroids in animal physiology.

Tags

Keywords: GonadectomyBlood SamplingJapanese MedakaTeleostOvariectomyOrchidectomyAnesthesiaIncisionSuturingBlood DrawCaudal Peduncle Vein

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62006?article_type=v

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
check_url/62006?article_type=v

Noninvasive In Vivo Small Animal MRI and MRS: Basic Experimental Procedures
check_url/62006?article_type=v

Microinjection of Medaka Embryos for use as a Model Genetic Organism
check_url/62006?article_type=v

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
check_url/62006?article_type=v

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
check_url/62006?article_type=v

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
check_url/62006?article_type=v

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation
check_url/62006?article_type=v

Non-Terminal Blood Sampling Techniques in Guinea Pigs
check_url/62006?article_type=v

Sampling Blood from the Lateral Tail Vein of the Rat
check_url/62006?article_type=v

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Read Article