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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus

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09:08 min

July 27, 2021

DOI:

09:08 min
July 27, 2021

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Summary

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For in-depth mechanistic analysis of the respiratory syncytial virus (RSV) RNA synthesis, we report a protocol of utilizing the chaperone phosphoprotein (P) for coexpression of the RNA-free nucleoprotein (N0) for subsequent in vitro assembly of the virus-specific nucleocapsids (NCs).

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