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Chapters
- 00:00Introduction
- 01:04Microscope Plate Setup
- 03:16Yeast Dilution
- 06:36Sonication (Optional)
- 08:18Microscope Setup
- 10:05Representative Results
- 11:00Experimental Pitfalls
- 12:22Conclusion
Yeast growth phenotypes are precisely measured through highly parallel time-lapse imaging of immobilized cells growing into microcolonies. Simultaneously, stress tolerance, protein expression, and protein localization can be monitored, generating integrated datasets to study how environmental and genetic differences, as well as gene-expression heterogeneity among isogenic cells, modulate growth.
