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Journal / Biology / Isolando e Imagens Ao Vivo, Regiões de Marcapasso …
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Biology

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Isolando e Imagens Ao Vivo, Regiões de Marcapasso Intacta da Pelve Renal do Rato por Vibratome Sectioning
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Isolando e Imagens Ao Vivo, Regiões de Marcapasso Intacta da Pelve Renal do Rato por Vibratome Sectioning

Article DOI: 10.3791/62040-v • 07:38 min • April 30th, 2021
April 30th, 2021 •
1,2, 1, 1,3
1Department of Physiology & Cell Biology, University of Nevada, Reno School of Medicine, 2Department of Physiology & Membrane Biology, University of California School of Medicine, 3Department of Life & Health Sciences, Dundalk Institute of Technology

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O objetivo deste protocolo é isolar regiões de marca-passo intactas da pelve renal do rato usando seção de vibratome. Essas seções podem então ser usadas para imagens in situ Ca2+ para elucidar propriedades transitórias ca2+ de células marcapassos e outras células intersticiais em fatias de vibratome.

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Biologia Questão 170 Pélvis Renal marca-passo célula intersticicular músculo liso trato urinário imagem Ca2+, urogenital rim PDGFRα Ano1
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