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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
 

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Article DOI: 10.3791/62045-v
March 26th, 2021

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Summary March 26th, 2021

This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.

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