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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

DOI:
10.3791/62045-v

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09:21 min

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March 26, 2021

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, , , , , , ,
1College of Optometry,University of Houston, 2Department of Biology,DePauw University, 3Center for Translational Research on Inflammatory Diseases (CTRID),Michael E. DeBakey Veterans Affairs Medical Center, 4Children’s Nutrition Center,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 01:05Tissue Processing
  • 04:19Block Preparation
  • 06:59Results: Representative Serial Block-Face Scanning Electron Microscopy (SBF-SEM) Imaging
  • 08:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol outlines a routine method for using serial block-face scanning electron microscopy (SBF-SEM), a powerful 3D imaging technique. Successful application of SBF-SEM hinges on proper fixation and tissue staining techniques, as well as careful consideration of imaging settings. This protocol contains practical considerations for the entirety of this process.

Tags

Keywords: Serial Block-Face Scanning Electron Microscopy (SBF-SEM)Heavy Metal StainingResin InfiltrationAutomated Image Capture

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